Strain engineering strategies for improving whole-cell biocatalysis: engineering Escherichia coli to overproduce xylitol as an example.

Abstract

This chapter provides an overview of key tools and methodologies available to practitioners of biocatalysis interested in using microorganisms to carry out biotransformations and describes specific examples of applying genetic modification strategies for strain design. We focus on the use of the polymerase chain reaction (PCR) for gene amplification… (More)
DOI: 10.1007/978-1-61779-132-1_15

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