Storage of cane toad (Bufo marinus) sperm for 6 days at 0 degrees C with subsequent cryopreservation.

@article{Browne2002StorageOC,
  title={Storage of cane toad (Bufo marinus) sperm for 6 days at 0 degrees C with subsequent cryopreservation.},
  author={Robert Kenneth Browne and Jeffery Jon Davis and M. Pomering and Jennifer Clulow},
  journal={Reproduction, fertility, and development},
  year={2002},
  volume={14 5-6},
  pages={
          267-73
        }
}
We investigated the recovery of motility of cane toad (Bufo marinus) sperm after storage for 6 days at 0 degree C (on ice) and after subsequent cryopreservation. Sperm suspensions were prepared from testes macerated in either simplified amphibian Ringer (SAR) or 10% (w/v) sucrose diluents, with 15% or 20% (v/v) glycerol or Me2SO as cryoprotectants, and were stored for 6 days. Alternatively, suspensions were prepared in either SAR or 10% (w/v) sucrose diluent and stored for 6 days, after which… CONTINUE READING