Stoichiometry of the Large Conductance Bacterial Mechanosensitive Channel of E. coli. A Biochemical Study

@article{Sukharev1999StoichiometryOT,
  title={Stoichiometry of the Large Conductance Bacterial Mechanosensitive Channel of E. coli. A Biochemical Study},
  author={Sergei Sukharev and Melanie J. Schroeder and Darrell R. McCaslin},
  journal={The Journal of Membrane Biology},
  year={1999},
  volume={171},
  pages={183-193}
}
MscL, a 15 kDa transmembrane protein, is the only component involved in the formation of a 3 nS channel in the inner membrane of Escherichia coli that opens in response to mechanical or osmotic stress. While previous data had suggested that the functional MscL complex might be a hexamer, a recent crystallographic study of the MscL homologue from M. tuberculosis reveals a pentameric structure. The present work further examines the stoichiometry of the E. coli MscL using a variety of biochemical… CONTINUE READING