Stoichiometry of the H+-ATPase of growing and resting, aerobic Escherichia coli.

@article{Kashket1982StoichiometryOT,
  title={Stoichiometry of the H+-ATPase of growing and resting, aerobic Escherichia coli.},
  author={Eva R. Kashket},
  journal={Biochemistry},
  year={1982},
  volume={21 22},
  pages={
          5534-8
        }
}
The H+/ATP stoichiometry of the proton-translocating ATPase was investigated in growing and nongrowing, respiring cells of Escherichia coli. The protonmotive force, delta p, was determined by measuring the transmembrane chemical gradient of protons, delta pH, from the cellular accumulation of benzoate anions, and the electrical gradient, delta psi, from the accumulation of the lipophilic cation tetraphenylphosphonium (TPP+). The accumulation of lactose was also used to calculate the delta p in… CONTINUE READING

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