Stoichiometric protein complex formation and over-expression using the prokaryotic native operon structure.

@article{Poulsen2010StoichiometricPC,
  title={Stoichiometric protein complex formation and over-expression using the prokaryotic native operon structure.},
  author={Christian Keis Poulsen and Simon J. Holton and Arie Geerlof and Matthias Wilmanns and Young-Hwa Song},
  journal={FEBS letters},
  year={2010},
  volume={584 4},
  pages={669-74}
}
In prokaryotes, operon encoded proteins often form protein-protein complexes. Here, we show that the native structure of operons can be used to efficiently overexpress protein complexes. This study focuses on operons from mycobacteria and the use of Mycobacterium smegmatis as an expression host. We demonstrate robust and correct stoichiometric expression of dimers to higher oligomers. The expression efficacy was found to be largely independent of the intergenic distances. The strategy was… CONTINUE READING

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