Stoichiometric analysis of oligomerization of membrane proteins on living cells using coiled-coil labeling and spectral imaging.

@article{Kawano2013StoichiometricAO,
  title={Stoichiometric analysis of oligomerization of membrane proteins on living cells using coiled-coil labeling and spectral imaging.},
  author={Kenichi Kawano and Yoshiaki Yano and Kaoru Omae and Sayaka Matsuzaki and Katsumi Matsuzaki},
  journal={Analytical chemistry},
  year={2013},
  volume={85 6},
  pages={3454-61}
}
Many membrane proteins are proposed to work as oligomers; however, the conclusion is sometimes controversial, as for β2-adorenergic receptor (β2AR), which is one of the best-studied family A G-protein-coupled receptors. This is due to the lack of methods for easy and precise detection of the oligomeric state of membrane proteins on living cells. Here, we show that a combination of the coiled-coil tag-probe labeling method and spectral imaging enable a stoichiometric analysis of the oligomeric… CONTINUE READING

Similar Papers

Loading similar papers…