Stimulus-secretion coupling of arginine-induced insulin release. Insulinotropic action of agmatine.

  title={Stimulus-secretion coupling of arginine-induced insulin release. Insulinotropic action of agmatine.},
  author={A. Sener and P. Lebrun and F. Blachier and W. Malaisse},
  journal={Biochemical pharmacology},
  volume={38 2},
Agmatine causes a concentration-related (0.1-1.0 mM) stimulation of insulin secretion by rat pancreatic islets exposed to D-glucose (5.6-11.1 mM) but, like L-arginine, fails to affect insulin release either in the absence of glucose or at a high concentration (16.7 mM) of the hexose. Agmatine, which accumulates in islet cells, also stimulates 45Ca net uptake by the islets. The secretory response to agmatine represents a delayed process. It is enhanced by theophylline and suppressed by antimycin… Expand
Stimulus‐secretion coupling of arginine‐induced insulin release: Significance of changes in extracellular and intracellular pH
It is suggested that the yet unidentified target for L‐arginine and its non‐metabolized analogue in islet cells displays pH‐dependency with optimal responsiveness at alkaline pH. Expand
Effects of the beta-carbolines, harmane and pinoline, on insulin secretion from isolated human islets of Langerhans.
The results reveal that beta-carbolines can potentiate the rate of insulin secretion from human islets and suggest that these agents may be useful prototypes for the development of novel insulin secretagogues. Expand
Homologous desensitization of pancreatic beta cells to glucose response by polyunsaturated fatty acids
The data suggest that a mixture of 10 M M linoleic acid and 5 m M linolenic acid can desensitize isolated perifused islets to glucose but not arginine, and glucose intolerance associated with lipemia may result, at least in part, from a direct effect on the islets of Langerhans. Expand
Clonidine-displacing substance and its putative role in control of insulin secretion: a minireview.
  • S. Chan
  • Biology, Chemistry
  • General pharmacology
  • 1998
Overall, CDS displays many characteristics expected of an endogenous regulator of insulin secretion acting through the islet beta-cell imidazoline site, which strengthens the hypothesis that the islets that were desensitized to the effects of the imdazoline secretagogue efaroxan were refractory to the actions of CDS. Expand
Agmatine inhibits arginine vasopressin-stimulated urea transport in the rat inner medullary collecting duct.
Agmatine inhibits AVP stimulated Pu by a cAMP-dependent mechanism and the possibility exists of an unknown agmatine-selective receptor modulating urea transport in the rat IMCD. Expand
The effect of the putative endogenous imidazoline receptor ligand, clonidine‐displacing substance, on insulin secretion from rat and human islets of Langerhans
The data suggest that CDS behaves as a potent endogenous insulin secretagogue acting at the islet imidazoline receptor, and is able to potentiate the insulin secretory response of rat islets incubated at 6 mm glucose, in a dose‐dependent manner. Expand
Electrophysiology of the -cell and mechanisms of inhibition of insulin release.
The sections in this article are: 1 Ion Channels in Insulin-Secreting Cells 1.1 Adenosine Triphosphate–Sensitive Potassium Channels 1.2 Extracellular Control of AdenosineExpand
Elevation of cytosolic calcium by imidazolines in mouse islets of Langerhans: implications for stimulus‐response coupling of insulin release
The present study shows that imidazolines will evoke rises in [Ca2+]i in intact islets, and this provides an explanation to account for the previously described effects of imidrazolines on KATP channels, the cell membrane potential and insulin secretion in pancreatic β‐cells. Expand
Amino Acids and Hormone Secretion in Pigs
Amino acids have been implicated in the regulation of hormone synthesis and secretion (Kuhara et al. 1991; Newsholme et al. 2005; Zhen et al. 2012). Amino acids stimulate or inhibit the release ofExpand
Transport and metabolism of agmatine in rat hepatocyte cultures.
Rat hepatocytes in culture take up [14C]-agmatine by both a high-Affinity transport system and a low-affinity system, and the rate of agmatine uptake is increased in cells deprived of polyamines with difluoromethylornithine. Expand


The stimulus-secretion coupling of glucose-induced insulin release. 3. Uptake of 45 calcium by isolated islets of Langerhans.
Although glucose failed to induce insulin release at low Ca++ concentration, the reduced uptake of calcium found in isolated islets of Langerhans paralleled those in insulin secretion by the islets. Expand
The role of calcium in glucagon release. Interactions between arginine and calcium.
Results clearly indicate that calcium is necessary for either the recognition or effectiveness of arginine as a stimulus to the alpha-cell, and it is suggested that calcium plays a versatile role - permissive, inhibitory and stimulatory - in glucagon secretion. Expand
The stimulus-secretion coupling of glucose-induced insulin release. VII. A proposed site of action for adenosine-3',5'-cyclic monophosphate.
It is concluded that the insulinotropic action of cAMP could be due to a glucose-independent translocation of calcium within the beta cell, from an organelle-bound pool to a cytoplasmic pool of ionized calcium readily available for transport across the cell membrane. Expand
Regulation of calcium fluxes in pancreatic islets: dissociation between calcium and insulin release.
It is concluded that this secondary rise, which originates from a lanthanum‐nondisplaceable calcium pool, does not correspond solely to an exocytotic release of 45calcium and could represent, in part at least, a displacement of 45Calcium from cellular sites and reflect a glucose‐induced increase in the rate of calcium entry in islet cells. Expand
An inhibitory role for polyamines in protein kinase C activation and insulin secretion in mouse pancreatic islets.
It is suggested that inhibition of protein kinase C by polyamines may be of significance for regulation of insulin secretion in vivo and that Ca2+ influx may function by displacing inhibitory polyamines bound to phosphatidylserine in membranes. Expand
Polyamines and insulin production in isolated mouse pancreatic islets.
It is concluded that putrescine and spermidine are necessary for the maintenance of normal insulin and protein biosynthesis, whereas spermine may exert a role in some other cellular processes, such as DNA replication, RNA transcription and glucose-stimulated insulin release. Expand
Determination of polyamines and related compounds by reversed-phase high-performance liquid chromatography: Improved separation systems
Abstract By employing a column with a high capacity factor and high resolution, separation of the ion pairs with octane sulphonic acid of the natural di- and polyamines, and related compounds, wasExpand
High-performance liquid chromatographic procedure for the simultaneous determination of the natural polyamines and their monoacetyl derivatives.
Octane sulfonate was used to form ion pairs with the polycations and acetylspermidine determinations directly from tissue extracts and body fluids without pre-purification. Expand