Stimulatory effects of propylthiouracil on pregnenolone production through upregulation of steroidogenic acute regulatory protein expression in rat granulosa cells.

  title={Stimulatory effects of propylthiouracil on pregnenolone production through upregulation of steroidogenic acute regulatory protein expression in rat granulosa cells.},
  author={Mei-Chih Chen and Shyi‐Wu Wang and S. Kan and S. Tsai and Yu-Ching Wu and Paulus S. Wang},
  journal={Toxicological sciences : an official journal of the Society of Toxicology},
  volume={118 2},
Propylthiouracil (PTU) is a common and effective clinical medicine for the treatment of hyperthyroidism. Our previous study demonstrated that short-term treatment with PTU inhibits progesterone production in rat granulosa cells. However, our present results indicate that a 16-h treatment with PTU was able to stimulate pregnenolone production in rat granulosa cells, although progesterone production was diminished by PTU through inhibition of 3β-hydroxysteroid dehydrogenase. Notably, we found… Expand
4 Citations
Immunolocalization of steroidogenic acute regulatory protein-related lipid transfer (START) domain-containing proteins in the developing cerebellum of normal and hypothyroid rats
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Direct effect of propylthiouracil on progesterone release in rat granulosa cells
The present results suggest that PTU decreases the progesterone release by granulosa cells via a thyroid‐independent mechanism involving the inhibition of post‐cAMP pathway, and the activities of intracellular calcium, steroidogenic enzyme, and StAR protein functions. Expand
Inhibition of Testosterone Production by Propylthiouracil in Rat Leydig Cells1
It is suggested that PTU acts directly on rat Leydig cells to diminish testosterone production by inhibiting P450scc function and StAR expression. Expand
Direct effects of propylthiouracil on testosterone production in monkeys.
The results suggest that PTU inhibits testosterone secretion via a mechanism independent of the secretion of T4 and LH in primates through a decreased activity of 17beta-hydroxysteroid dehydrogenase (17beta-HSD) and post-cAMP pathways. Expand
Differential effects of nonylphenol on testosterone secretion in rat Leydig cells.
In vitro experiments revealed that NP alone stimulated testosterone release through increase of both protein levels and activities of the StAR and P450(SCC), but inhibited hCG-induced testosterone release in rat Leydig cells, suggesting that NP had differential effects on testosterone synthesis. Expand
Transcriptional regulation of the rat steroidogenic acute regulatory protein gene by steroidogenic factor 1.
It is demonstrated that cAMP administration to rat luteal cells stimulates expression of both StAR messenger RNA and protein, and the two proximal SF-1 binding sites are crucial for StAR gene transcription, both at a basal level and in response to cAMP stimulation. Expand
Interferon-gamma inhibits the steroidogenic acute regulatory protein messenger ribonucleic acid expression and protein levels in primary cultures of rat Leydig cells.
IFNgamma inhibits Leydig cell steroidogenesis by down-regulating StAR gene expression and protein production. Expand
Pharmacological effects of propylthiouracil on corticosterone secretion in male rats.
  • M. Lo, S. W. Wang, +5 authors P. S. Wang
  • Chemistry, Medicine
  • Journal of investigative medicine : the official publication of the American Federation for Clinical Research
  • 1998
Results suggest that PTU counteracts both basal and ACTH-induced adrenal steroidogenesis through their attenuation of the activity of 11 beta-hydroxylase and cAMP production in rat ZFR cells. Expand
Direct inhibitory effect of digitalis on progesterone release from rat granulosa cells
The present results suggest that digoxin and digitoxin decrease the progesterone release by granulosa cells via a Na+,K+‐ATPase‐independent mechanism involving the inhibition of post‐cyclic AMP pathway, cytochrome P450scc and StAR protein functions. Expand
cAMP-independent signaling regulates steroidogenesis in mouse Leydig cells in the absence of StAR phosphorylation.
It is demonstrated that, in mouse Leydig cells, cAMP-independent signaling events regulate steroidogenesis in a StAR phosphorylation-independent manner. Expand
Inhibition of salmon calcitonin on secretion of progesterone and GnRH-stimulated pituitary luteinizing hormone.
It is suggested that sCT inhibits progesterone production in rats by preventing the stimulatory effect of GnRH on LH release in rat APs and acting directly on ovarian granulosa cells to decrease the activities of post-cAMP pathway and steroidogenic enzymes. Expand