Stimulatory effect of genistein and daidzein on protein synthesis in osteoblastic MC3T3-E1 cells: Activation of aminoacyl-tRNA synthetase

@article{Yamaguchi2000StimulatoryEO,
  title={Stimulatory effect of genistein and daidzein on protein synthesis in osteoblastic MC3T3-E1 cells: Activation of aminoacyl-tRNA synthetase},
  author={Masayoshi Yamaguchi and Emi Sugimoto},
  journal={Molecular and Cellular Biochemistry},
  year={2000},
  volume={214},
  pages={97-102}
}
The effect of genistein and daidzein on protein synthesis in osteoblastic MC3T3-E1 cells in vitro was investigated to determine a cellular mechanism by which the isoflavones stimulate bone formation. Cells were cultured for 48 h in α-minimal essential medium containing either vehicle, genistein (10–7–10–5 M) or daidzein (10–7–10–5 M). The 5,500 g supernatant of cell homogenate was used for assay of protein synthesis with [3H]leucine incorporation in vitro. The culture with genistein or daidzein… CONTINUE READING

8 Figures & Tables

Connections & Topics

Mentioned Connections BETA
The effect of genistein and daidzein on protein synthesis in osteoblastic MC3T3-E1 cells in vitro was investigated to determine a cellular mechanism by which the isoflavones stimulate bone formation .
Moreover , when genistein ( 10–7–10–5 M ) or daidzein ( 10–6 and 10–5 M ) was added to the reaction mixture containing the cell homogenate obtained from osteoblastic cells cultured without isoflavone , protein synthesis was significantly raised .
The effect of genistein ( 10–5 M ) or daidzein ( 10–5 M ) in elevating protein synthesis was significantly prevented , when cells were cultured for 48 h in a medium containing either actinomycin D ( 10–7 M ) or cycloheximide ( 10–6 M ) in the absence or presence of isoflavones .
The effect of genistein and daidzein on protein synthesis in osteoblastic MC3T3-E1 cells in vitro was investigated to determine a cellular mechanism by which the isoflavones stimulate bone formation .
Moreover , when genistein ( 10–7–10–5 M ) or daidzein ( 10–6 and 10–5 M ) was added to the reaction mixture containing the cell homogenate obtained from osteoblastic cells cultured without isoflavone , protein synthesis was significantly raised .
The effect of genistein ( 10–5 M ) or daidzein ( 10–5 M ) in elevating protein synthesis was significantly prevented , when cells were cultured for 48 h in a medium containing either actinomycin D ( 10–7 M ) or cycloheximide ( 10–6 M ) in the absence or presence of isoflavones .
Moreover , when genistein ( 10–7–10–5 M ) or daidzein ( 10–6 and 10–5 M ) was added to the reaction mixture containing the cell homogenate obtained from osteoblastic cells cultured without isoflavone , protein synthesis was significantly raised .
The effect of genistein ( 10–5 M ) or daidzein ( 10–5 M ) in elevating protein synthesis was significantly prevented , when cells were cultured for 48 h in a medium containing either actinomycin D ( 10–7 M ) or cycloheximide ( 10–6 M ) in the absence or presence of isoflavones .
The effect of genistein and daidzein on protein synthesis in osteoblastic MC3T3-E1 cells in vitro was investigated to determine a cellular mechanism by which the isoflavones stimulate bone formation .
Moreover , when genistein ( 10–7–10–5 M ) or daidzein ( 10–6 and 10–5 M ) was added to the reaction mixture containing the cell homogenate obtained from osteoblastic cells cultured without isoflavone , protein synthesis was significantly raised .
The effect of genistein and daidzein on protein synthesis in osteoblastic MC3T3-E1 cells in vitro was investigated to determine a cellular mechanism by which the isoflavones stimulate bone formation .
The effect of genistein ( 10–5 M ) or daidzein ( 10–5 M ) in elevating protein synthesis was significantly prevented , when cells were cultured for 48 h in a medium containing either actinomycin D ( 10–7 M ) or cycloheximide ( 10–6 M ) in the absence or presence of isoflavones .
Moreover , when genistein ( 10–7–10–5 M ) or daidzein ( 10–6 and 10–5 M ) was added to the reaction mixture containing the cell homogenate obtained from osteoblastic cells cultured without isoflavone , protein synthesis was significantly raised .
The effect of genistein and daidzein on protein synthesis in osteoblastic MC3T3-E1 cells in vitro was investigated to determine a cellular mechanism by which the isoflavones stimulate bone formation .
The effect of genistein ( 10–5 M ) or daidzein ( 10–5 M ) in elevating protein synthesis was significantly prevented , when cells were cultured for 48 h in a medium containing either actinomycin D ( 10–7 M ) or cycloheximide ( 10–6 M ) in the absence or presence of isoflavones .
All Topics