Stimulation of troutCYP1A gene expression in mouse HEPA-1 cells by 3-methylcholanthrene

@article{Lee1997StimulationOT,
  title={Stimulation of troutCYP1A gene expression in mouse HEPA-1 cells by 3-methylcholanthrene},
  author={So Young Lee and Yhun Yhong Sheen},
  journal={Archives of Pharmacal Research},
  year={1997},
  volume={20},
  pages={404-409}
}
TroutCYP1A-CAT expression construct was generated by cloning ∼3.5 Kb 5′ flanking DNA of trout liverCYP1A gene in front of CAT gene at pCAT-basic vector. Hepa 1 cells, which are known to contain a functional arylhydrocarbon receptor' were transfected with troutCYP1A-CAT using lipofectin. 3-Methylcholanthrene (1 nM) was added into hepa 1 cells in culture in order to examine if 5′ flanking DNA of troutCYP1A gene could interact with mouse transactivating factors to bring about transcription of the… CONTINUE READING