Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS.

@article{Agarwal1991StimulationOT,
  title={Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS.},
  author={Kan Agarwal and Kyuwon Baek and Choon Ju Jeon and Katsushiro Miyamoto and Akihiko Ueno and Ha Sik Yoon},
  journal={Biochemistry},
  year={1991},
  volume={30 31},
  pages={7842-51}
}
The eukaryotic transcriptional factor TFIIS enhances transcript elongation by RNA polymerase II. Here we describe two functional domains in the 280 amino acid human TFIIS protein: residues within positions 100-230 are required for binding to polymerase, and residues 230-280, which form a zinc finger, are required in conjunction with the polymerase binding region for transcriptional stimulation. Interestingly, a mutant TFIIS with only the polymerase binding domain actually inhibits transcription… CONTINUE READING