Stimulation of tolbutamide hydroxylation by acetone and acetonitrile in human liver microsomes and in a cytochrome P-450 2C9-reconstituted system.

@article{Palamanda2000StimulationOT,
  title={Stimulation of tolbutamide hydroxylation by acetone and acetonitrile in human liver microsomes and in a cytochrome P-450 2C9-reconstituted system.},
  author={Jairam R. Palamanda and Wang Wei Feng and Charles Chia-Lee Lin and Amin A. Nomeir},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2000},
  volume={28 1},
  pages={38-43}
}
Organic solvents are often used to solubilize lipophilic new chemical entities before their addition to in vitro test systems such as microsomal stability or cytochrome P-450 (CYP) inhibition. However, the effect of these organic solvents on the test systems is not usually characterized. This study was initiated to evaluate the effect of acetonitrile and acetone, in addition to other organic solvents, on the tolbutamide hydroxylation activity of CYP2C9 in both human liver microsomes and a… CONTINUE READING
12 Citations
25 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 12 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 25 references

Effect of methanol , ethanol , dimethyl sulfoxide , and acetonitrile on in vitro activities of cDNA - expressed human cytochromes P - 450

  • N Chauret, A Gauthier, DA Nicoll-Griffith
  • Drug Metab Dispos
  • 1999

Effect of methanol, ethanol, dimethyl sulfoxide, and acetonitrile on in vitro activities of cDNA-expressed human cytochromes P-450.Drug Metab Dispos27:246–249

  • WF JrBusby, JM Ackermann, CL Crespi
  • 1999

Accelerated communication : Effect of common organic solvents on in vitro CYP 450 mediated metabolic activities in human liver microsomes

  • CL Crespi, VP Miller
  • Drug Metab Dispos
  • 1998

Evaluation of the selectivity of in vitro probes and suitability of organic solvents for the measurement of human cytochrome P450 monooxygenase activities

  • J Wang, Y Wang, JD Unadkat
  • Drug Metab Dispos
  • 1998
3 Excerpts

Validation of a rapid microtiter plate assay to conduct cytochrome P4502D6 enzyme inhibition studies

  • J 851180–1185. Palamanda, LV Favreau, CC Lin, AA Nomeir
  • Drug Discovery Today
  • 1998
1 Excerpt

Similar Papers

Loading similar papers…