Stimulation of the human RAD51 nucleofilament restricts HIV-1 integration in vitro and in infected cells.

@article{Cosnefroy2012StimulationOT,
  title={Stimulation of the human RAD51 nucleofilament restricts HIV-1 integration in vitro and in infected cells.},
  author={Oph{\'e}lie Cosnefroy and Alice Tocco and P. Lesbats and Sylvain Thierry and Christina Calmels and Tatiana Wiktorowicz and Sandrine Reigadas and Yong-kuk Kwon and Anne De Cian and S{\'e}bastien Desfarges and P Bonot and Joseph San Filippo and Simon Litvak and Eric Le Cam and Axel Rethwilm and H{\'e}rv{\'e} Fleury and Philip P. Connell and Phillip Sung and Olivier Delelis and Marie Line Andreola and Vincent Parissi},
  journal={Journal of virology},
  year={2012},
  volume={86 1},
  pages={513-26}
}
Stable HIV-1 replication requires the DNA repair of the integration locus catalyzed by cellular factors. The human RAD51 (hRAD51) protein plays a major role in homologous recombination (HR) DNA repair and was previously shown to interact with HIV-1 integrase (IN) and inhibit its activity. Here we determined the molecular mechanism of inhibition of IN. Our standard in vitro integration assays performed under various conditions promoting or inhibiting hRAD51 activity demonstrated that the… CONTINUE READING