Stimulation of secretion by the T84 colonic epithelial cell line with dietary flavonols.

  title={Stimulation of secretion by the T84 colonic epithelial cell line with dietary flavonols.},
  author={T. D. Nguyen and Andrew T. Canada and Greg Heintz and Thomas W. Gettys and Jonathan A. Cohn},
  journal={Biochemical pharmacology},
  volume={41 12},

Dietary flavonol quercetin induces chloride secretion in rat colon.

It is demonstrated that the dietary flavonol quercetin induces Cl- secretion and most likely HCO-3 secretion in rat small and large intestine.

The Secretory Response of the Rat Colon to the Flavonol Quercetin is Dependent on Ca2+‐Calmodulin

Results indicate that the chloride secretion induced by quercetin in rat colon depends on Ca2+ and calmodulin, and the cAMP pathway and inhibition of phosphodiesterase appear not to be responsible for the secretory activity of the flavonol.

Effect of the flavonol quercetin on ion transport in the isolated intestine of the eel, Anguilla anguilla.

The Chemopreventive Agent Resveratrol Stimulates Cyclic AMP–Dependent Chloride Secretion In vitro

The main mechanism of action of resveratrol in intestinal epithelia is cAMP-induced chloride secretion which can be suppressed by butyrate, suggesting that in cancer chemoprevention, both agents should be combined to reduce an undesired side effect such as diarrhea and to benefit from the known agonistic effect of both agents on differentiation of colon cancer cells.

Flavonoids stimulate Cl conductance of human airway epithelium in vitro and in vivo.

The ability of the flavonoids genistein, apigenin, kaempferol, and quercetin to activate cystic fibrosis transmembrane conductance regulator-mediated Cl currents in human airway epithelium was investigated and data imply that the activation kinetics and the binding site(s) for flavonoid were significantly altered by forskolin stimulation.

Nobiletin Stimulates Chloride Secretion in Human Bronchial Epithelia via a cAMP/PKA-Dependent Pathway

The mechanisms involved activation of adenylate cyclase- and cAMP/PKA-dependent pathways, leading to activation of apical CFTR Cl- channels, and stimulated transepithelial Cl- secretion across human bronchial epithelia.

The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer.

Western medicine has not yet used flavonoids therapeutically, even though their safety record is exceptional, and suggestions are made where such possibilities may be worth pursuing.

Actions of Quercetin, a Polyphenol, on Blood Pressure

Novel, unique mechanisms of quercetin’s anti-hypertensive action via activation of NKCC1 are introduced in detail.



The toxicity of flavonoids to guinea pig enterocytes.

Immune-related intestinal chloride secretion. II. Effect of adenosine on T84 cell line.

The results suggest that either adenosine mobilizes an intracellular pool of cAMP that is extremely efficiently coupled to the cAMP-dependent protein kinase and is thereafter rapidly destroyed or that second messenger(s) other than cAMP, cGMP, or Ca2+ are able to activate Cl- secretion in the T84 cell line.

Mechanism of chloride secretion induced by carbachol in a colonic epithelial cell line.

Carbachol's effect on Cl- secretion is greatly augmented in the presence of VIP or PGE1, which open a cAMP-sensitive Cl- channel on the apical membrane, accounting for a potentiated response.

Chloride secretory mechanism induced by prostaglandin E1 in a colonic epithelial cell line.

The findings indicate that the prostaglandin E1-stimulated Cl- secretion, which is associated with an increase in cyclic AMP level, intimately involves a bumetanide-sensitive Na+,K+,Cl- cotransport pathway that serves as a Cl- uptake step across the basolateral membrane.

Vasoactive intestinal peptide stimulates protein phosphorylation in a colonic epithelial cell line.

  • J. Cohn
  • Biology, Chemistry
    The American journal of physiology
  • 1987
The T84 colonic epithelial cell line was used to examine protein phosphorylation during neurohumoral stimulation of ion transport and suggests that T84 cells offer a useful model for studying the possibility that such phosphorylated events regulate enterocyte ion transport.

The production of hydroxyl and semiquinone free radicals during the autoxidation of redox active flavonoids.

It was demonstrated that both the respiratory bursts and the autoxidation result in the production of superoxide (O2‒) and hydrogen peroxide (H2O2) in isolated beef heart mitochondrial preparations.

Immune system control of rat and rabbit colonic electrolyte transport. Role of prostaglandins and enteric nervous system.

Results indicate that immune system agonists alter intestinal epithelial electrolyte transport through release of cyclooxygenase products from cells in the lamina propria with at least 50% of the response being due to cyclo oxygengenase product activation of the enteric nervous system.