Stimulation of nitric oxide synthase during oxidative endothelial cell injury.

  title={Stimulation of nitric oxide synthase during oxidative endothelial cell injury.},
  author={Shunichi Shimizu and Masahiro Nomoto and Shinsaku Naito and Toshinori Yamamoto and Kazutaka Momose},
  journal={Biochemical pharmacology},
  volume={55 1},
The purpose of this study was to determine changes in nitric oxide synthase (NOS) activity during the process of lethal oxidative cell injury following H2O2 treatment of endothelial cells. NOS activity was determined by measuring the conversion of [3H]arginine ([3H]Arg) to [3H]citrulline ([3H]Cit). Cell death was assessed by measuring the release of intracellular lactate dehydrogenase (LDH). Moreover, cell death and changes in cytosolic free Ca2+ (Ca(i)2+) were measured simultaneously using a… CONTINUE READING
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