Stimulation of nerve growth factor biosynthesis in developing rat brain by reserpine: steroids as potential mediators.

@article{Fabrazzo1991StimulationON,
  title={Stimulation of nerve growth factor biosynthesis in developing rat brain by reserpine: steroids as potential mediators.},
  author={M Fabrazzo and Erminio Costa and Italo Mocchetti},
  journal={Molecular pharmacology},
  year={1991},
  volume={39 2},
  pages={144-9}
}
The stimulation of beta-adrenergic receptors by isoproterenol increases nerve growth factor (NGF) biosynthesis in C6 rat glioma cells, suggesting that norepinephrine may regulate NGF biosynthesis in vivo. We have tested this hypothesis in 21-day-old rats by depleting catecholamine stores with reserpine. Northern blot analysis of NGF mRNA, in combination with a two-site enzyme immunoassay for NGF, showed that depletion of catecholamines was associated with a 3-fold increase in NGF mRNA, which… CONTINUE READING