Stimulation of homology-directed gene targeting at an endogenous human locus by a nicking endonuclease

Abstract

Homologous recombination (HR) is a highly accurate mechanism of DNA repair that can be exploited for homology-directed gene targeting. Since in most cell types HR occurs very infrequently (approximately 10(-6) to 10(-8)), its practical application has been largely restricted to specific experimental systems that allow selection of the few cells that become… (More)
DOI: 10.1093/nar/gkp643

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@inproceedings{Nierop2009StimulationOH, title={Stimulation of homology-directed gene targeting at an endogenous human locus by a nicking endonuclease}, author={Gijsbert P. van Nierop and Antoine A. F. de Vries and Maarten Holkers and Krijn R. Vrijsen and Manuel A F V Gonçalves}, booktitle={Nucleic acids research}, year={2009} }