Stimulation-associated redistribution of H+-K+-ATPase activity in isolated gastric glands.

@article{Urushidani1987StimulationassociatedRO,
  title={Stimulation-associated redistribution of H+-K+-ATPase activity in isolated gastric glands.},
  author={Tetsuro Urushidani and J. L. Garc{\'i}a-Trevijano Forte},
  journal={The American journal of physiology},
  year={1987},
  volume={252 4 Pt 1},
  pages={
          G458-65
        }
}
The objective of this work is to establish a procedure to study the stimulation-dependent membrane redistribution and properties of H+-K+-ATPase in an in vitro model system, rabbit isolated gastric glands. Stimulated (10(-4) M histamine plus 10(-5) M forskolin) and resting (10(-4) M metiamide) glands were homogenized and fractionated into PO (40 g, 5 min), P1 (400 g, 10 min), P2 (14,500 g, 10 min), P3 (48,200 g, 90 min), and supernatant, S3. Significant changes occurred in the distribution of… CONTINUE READING
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