Steroid transformation at high substrate concentrations using immobilized Corynebacterium simplex cells

@article{Constantinides1980SteroidTA,
  title={Steroid transformation at high substrate concentrations using immobilized Corynebacterium simplex cells},
  author={Alkis Constantinides},
  journal={Biotechnology and Bioengineering},
  year={1980},
  volume={22}
}
The steroid transformation of hydrocortisone to prednisolone, combining the two techniques of immobilized whole cells and high steroid concentrations, was investigated and found to be a feasible process. Freeze‐dried Corynebacterium simplex cells were immobilized in collagen, tanned with glutaraldehyde, and cast into a membrane. The reaction was studied at hydrocortisone concentrations ranging from 5 to 50 mg/ml. The following aspects of the system were examined: (1) the substrate concentration… 
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