Steroid structural requirements for high affinity binding to human sex steroid binding protein (SBP)

  title={Steroid structural requirements for high affinity binding to human sex steroid binding protein (SBP)},
  author={Glenn R. Cunningham and Donald J. Tindall and Thomas J. Lobl and J. Allan Campbell and Anthony Means},
Steroid-binding and dimerization domains of human sex hormone-binding globulin partially overlap: steroids and Ca2+ stabilize dimer formation.
Evidence is provided that residues 134-139 influence the recognition of specific A/B ring conformations of steroid ligands and may constitute part of the steroid-binding domain and that substitutions at residues 138-148 impair dimerization.
Sex hormone-binding globulin/androgen-binding protein: Steroid-binding and dimerization domains
Profiles of ligands of sex hormone binding globulin in human serum.
  • B. Murphy
  • Medicine, Biology
    Journal of steroid biochemistry
  • 1988
Steroid interactions with cytochrome P-450 from testis microsomes.
Sex hormone-binding globulin is synthesized in target cells.
This work has reviewed the current state of knowledge on the SHBG gene and the role of SHBG in steroid signaling and addressed its function as a plasma-binding protein.
Relationship between ligand structure and affinity for androgen binding protein
The sequelae of events suggested that ABP may play a role in the androgen-dependent maturation of epididymal spermatozoa and male fertility in general.


Structural Requirements for Steroid Binding to Androgen Binding Proteins
Examining the steroid specificity of binding to a large number of steroidal compounds in order to characterize the binding sites of these binding proteins and to determine their binding specificities suggested that a fundamental difference exists between the binding Sites of ABP and CR.
Transport of steroid hormones: interaction of 70 drugs with testosterone-binding globulin and corticosteroid-binding globulin in human plasma.
The results indicate that binding to steroid transport proteins should be considered among the in vivo effects of drugs on endogenous steroid hormone levels.
An androgen binding protein in the testicular cytosol of human testis. Comparison with human plasma testosterone-estrogen binding globulin.
  • A. Hsu, P. Troen
  • Biology, Medicine
    The Journal of clinical investigation
  • 1978
Differences between hABP and TeBG, plus the reduction of plasma contamination as marked by albumin, suggest that the human mature testis contains an androgen binding protein separate from that circulating in plasma.
Multiple Forms of Rat Testicular Androgen Binding Protein: Physicochemical Properties and the Effect of Ca++
The presence of two forms of androgen binding protein (ABP) was confirmed in rat testicular cytosol and the order of binding affinity was dihydrotestosterone > testosterone > estradiol-17β >progesterone.
Studies of testosterone binding globulin.
  • T. Kato, R. Horton
  • Biology, Medicine
    The Journal of clinical endocrinology and metabolism
  • 1968
Testosterone binding globulin (TBG) activity tested by dextran coated charcoal particle adsorption of free labeled testosterone is increased in pregnancy or estrogen administration and appears slightly more active in normal female than male plasma.
Influence of purified plasma proteins on testosterone uptake and metabolism by normal and hyperplastic human prostate in "constant-flow organ culture".
The androstanolone/testosterone ratio in the prostate explants was critically dependent upon the SBP concentration in the superfusion medium, and it is suggested that, independent of its effect on the binding of testosterone, SBP has a direct effect on testosterone uptake and metabolism by the human prostate.
Androgen Binding Proteins and Androgen Levels in the Human Testis and Epididymis
Simultaneous assays of ABP and corticosteroid binding globulin in the same testicular extracts suggest that androgen binding to the second component could be readily explained by contamination with blood TeBG rather than by the existence of a testis specific ABP.