Steroid metabolism in teleost gonads: purification and identification of metabolites by high-performance liquid chromatography.

@article{Venkatesh1992SteroidMI,
  title={Steroid metabolism in teleost gonads: purification and identification of metabolites by high-performance liquid chromatography.},
  author={Byrappa Venkatesh and Chern Huay Tan and David E. Kime and T. J. Lam},
  journal={Steroids},
  year={1992},
  volume={57 6},
  pages={276-81}
}
A simple, efficient, and comprehensive technique for the purification, identification, and quantitation of the common steroid metabolites synthesized by the gonads of teleosts involving five systems of high-performance liquid chromatography (HPLC) was developed. Steroid standards were identified in HPLC by UV absorption at 254 nm or 280 nm, by differential refractive index, or by using radioactive standards. Metabolites that do not absorb UV light and are not resolved in the isocratic HPLC… CONTINUE READING