Steroid Effects on Cell Proliferation , Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells

@inproceedings{Lee2007SteroidEO,
  title={Steroid Effects on Cell Proliferation , Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells},
  author={Eun Ju Lee and Jinho Choi and Jin Hee Hyun and Kyung-Hyun Cho and Inho Hwang and Hyun-Jeong Lee and Jongsoo Chang and Inho Choi},
  year={2007}
}
The present study was conducted to establish primary bovine muscle satellite cell (MSC) culture conditions and to investigate the effects of various steroid hormones on transcription of the genes involved in muscle cell proliferation and differentiation. Of three different types of proteases (type II collagenase, pronase and trypsin-EDTA) used to hydrolyze the myogenic satellite cells from muscle tissues, trypsin-EDTA treatment yielded the highest number of cells. The cells separated by… CONTINUE READING
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