Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.

@article{Botstein1979SterileHY,
  title={Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.},
  author={David Botstein and S. Carl Falco and Susan E Stewart and Murray E Brennan and Stewart Scherer and Dan T. Stinchcomb and Kevin Struhl and Ronald W. Davis},
  journal={Gene},
  year={1979},
  volume={8 1},
  pages={17-24}
}
A system of biological containment for recombinant DNA experiments in Saccharomyces cerevisiae (Brewer's/Baker's yeast) is described. The principle of containment is sterility: the haploid host strains all contain a mating-type-non-specific sterile mutation. The hosts also contain four auxotrophic mutations suitable for selection for the various kinds of… CONTINUE READING