Steric hindrance regulation of the Pseudomonas aeruginosa amidase operon.

  title={Steric hindrance regulation of the Pseudomonas aeruginosa amidase operon.},
  author={Richard P. A. Norman and Chit Laa Poh and Laurence H. Pearl and Brendan P O'Hara and Robert E. Drew},
  journal={The Journal of biological chemistry},
  volume={275 39},
Expression of the amidase operon of Pseudomonas aeruginosa is controlled by AmiC, the ligand sensor and negative regulator, and AmiR the transcription antitermination factor activator. We have titrated out AmiC repression activity in vivo by increased AmiR production in trans and shown AmiC regulation of the antitermination activity of AmiR by a steric hindrance mechanism. In the presence of the co-repressor butyramide we have isolated a stable AmiC.AmiR complex. Addition of the inducing ligand… CONTINUE READING

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