Stereospecific micellar electrokinetic chromatography assay of methionine sulfoxide reductase activity employing a multiple layer coated capillary.

@article{Zhu2013StereospecificME,
  title={Stereospecific micellar electrokinetic chromatography assay of methionine sulfoxide reductase activity employing a multiple layer coated capillary.},
  author={Qingfu Zhu and Rabab G El-Mergawy and Stefan H. Heinemann and Roland Sch{\"o}nherr and Pavel J{\'a}{\vc} and Gerhard K E Scriba},
  journal={Electrophoresis},
  year={2013},
  volume={34 18},
  pages={2712-7}
}
A micellar electrokinetic chromatography method for the analysis of the l-methionine sulfoxide diastereomers employing a successive multiple ionic-polymer layer coated fused-silica capillary was developed and validated in order to investigate the stereospecificity of methionine sulfoxide reductases. The capillary coating consisted of a first layer of hexadimethrine and a second layer of dextran sulfate providing a stable strong cathodic EOF and consequently highly repeatable analyte migration… CONTINUE READING