Stereoselectivity of lipases in supercritical carbon dioxide. I. Dependence of the regio- and enantioselectivity of porcine pancreas lipase on the water content during the hydrolysis of triolein and its partial glycerides.

@article{Glowacz1996StereoselectivityOL,
  title={Stereoselectivity of lipases in supercritical carbon dioxide. I. Dependence of the regio- and enantioselectivity of porcine pancreas lipase on the water content during the hydrolysis of triolein and its partial glycerides.},
  author={G Glowacz and M Bariszlovich and Manuela Linke and Peter G. Richter and Charles Fuchs and J T M{\"o}rsel},
  journal={Chemistry and physics of lipids},
  year={1996},
  volume={79 2},
  pages={101-6}
}
The stereoselectivity of porcine pancreas lipase (PPL) was investigated during the enzymatic hydrolysis of triolein and its partial glycerides in the presence of supercritical carbon dioxide (SCCO2) as reaction medium. The water content of the immobilized lipases was varied. The partial glycerides were separated into mono- and diglycerides by TLC, converted to their 3,5-dinitrophenylurethane derivatives and subsequently resolved into sn-1,2 and sn-2,3 enantiomers (estimation of dioleins) or… CONTINUE READING

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The partial glycerides were separated into mono- and diglycerides by TLC , converted to their 3,5-dinitrophenylurethane derivatives and subsequently resolved into sn-1,2 and sn-2,3 enantiomers ( estimation of dioleins ) or into sn-1 and sn-3 enantiomers ( estimation of monooleins ) by HPLC on a chiral stationary phase ( Sumichiral OA-4100 ) .
The partial glycerides were separated into mono- and diglycerides by TLC , converted to their 3,5-dinitrophenylurethane derivatives and subsequently resolved into sn-1,2 and sn-2,3 enantiomers ( estimation of dioleins ) or into sn-1 and sn-3 enantiomers ( estimation of monooleins ) by HPLC on a chiral stationary phase ( Sumichiral OA-4100 ) .
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