Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature

@article{Chaloupkova2011StereoselectivityAC,
  title={Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature},
  author={Radka Chaloupkova and Zbyněk Prokop and Yukari Sato and Yuji Nagata and Jiř{\'i} Damborsk{\'y}},
  journal={The FEBS Journal},
  year={2011},
  volume={278}
}
The effect of pH and temperature on structure, stability, activity and enantioselectivity of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110 was investigated in this study. Conformational changes have been assessed by circular dichroism spectroscopy, functional changes by kinetic analysis, while quaternary structure was studied by gel filtration chromatography. Our study shows that the DbjA enzyme is highly tolerant to pH changes. Its secondary and tertiary structure was not… Expand
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