Stereoscopic observation of enzyme distribution in lowicryl K4M-embedded specimens by conventional electron microscopy.

Abstract

The present investigation was undertaken to explore the value of the Lowicryl K4M embedding technique for enzyme histochemical examination of semi-thin sections. The low-temperature embedding procedure with Lowicryl K4M was found to provide favorable conditions for preservation of enzyme activity in tissue samples. We tested the histological effects of various fixatives; the best results were obtained using 4% paraformaldehyde when testing for AcPase, AlPase, TPPase, and Mg-ATPase in the dorsal root ganglion. The three-dimensional cellular fine structure could be clearly seen in stereo pair pictures under stereoscopy.

Cite this paper

@article{Tomiyasu1990StereoscopicOO, title={Stereoscopic observation of enzyme distribution in lowicryl K4M-embedded specimens by conventional electron microscopy.}, author={K. Tomiyasu and Fumio Nasu and Hiroaki Igarashi and Katsuichi Inomata}, journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society}, year={1990}, volume={38 4}, pages={595-9} }