Stereochemistry and mechanism of the conversion of 5-aminolaevulinic acid into porphobilinogen catalysed by porphobilinogen synthase.

Abstract

(3R)- and (3S)-Deuteriated forms of 5-aminolaevulinic acid have been synthesized and the (3R)-form shows a significantly larger isotope effect when incubated with porphobilinogen synthase from bovine liver and from Bacillus subtilis; based on this and on available crystal structures, a modified mechanism for the enzymic reaction is proposed.

Cite this paper

@article{Goodwin2003StereochemistryAM, title={Stereochemistry and mechanism of the conversion of 5-aminolaevulinic acid into porphobilinogen catalysed by porphobilinogen synthase.}, author={Catherine E Goodwin and Finian J Leeper}, journal={Organic & biomolecular chemistry}, year={2003}, volume={1 9}, pages={1443-6} }