Stereochemical outcome and kinetic effects of Rp- and Sp-phosphorothioate substitutions at the cleavage site of vaccinia type I DNA topoisomerase.

@article{Stivers2000StereochemicalOA,
  title={Stereochemical outcome and kinetic effects of Rp- and Sp-phosphorothioate substitutions at the cleavage site of vaccinia type I DNA topoisomerase.},
  author={James T Stivers and G Jayashree Jagadeesh and Barbara Nawrot and Wojciech J. Stec and Stewart Shuman},
  journal={Biochemistry},
  year={2000},
  volume={39 18},
  pages={5561-72}
}
To probe the mechanism of the reversible DNA phosphodiester bond cleavage and religation mechanism of the type I topoisomerase from vaccinia virus, we have synthesized DNA substrates carrying a single nonbridging Rp- or Sp-phosphorothioate (Ps) modification at the scissile phosphodiester (Pd) bond. Analysis of the stereochemical outcome of the net cleavage and rejoining reaction established that the reaction proceeds with retention of configuration, as expected for a double-displacement… CONTINUE READING
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