Stepwise loss of fluorescent core protein V from human adenovirus during entry into cells.

@article{Puntener2011StepwiseLO,
  title={Stepwise loss of fluorescent core protein V from human adenovirus during entry into cells.},
  author={Daniel Puntener and Martin F Engelke and Zsolt Ruzsics and Sten Strunze and Corinne Wilhelm and Urs F. Greber},
  journal={Journal of virology},
  year={2011},
  volume={85 1},
  pages={481-96}
}
Human adenoviruses (Ads) replicate and assemble particles in the nucleus. They organize a linear double-strand DNA genome into a condensed core with about 180 nucleosomes, by the viral proteins VII (pVII), pX, and pV attaching the DNA to the capsid. Using reverse genetics, we generated a novel, nonconditionally replicating Ad reporter by inserting green fluorescent protein (GFP) at the amino terminus of pV. Purified Ad2-GFP-pV virions had an oversized complete genome and incorporated about 38… CONTINUE READING