Stepwise isotope editing of [FeFe]-hydrogenases exposes cofactor dynamics.


The six-iron cofactor of [FeFe]-hydrogenases (H-cluster) is the most efficient H2-forming catalyst in nature. It comprises a diiron active site with three carbon monoxide (CO) and two cyanide (CN(-)) ligands in the active oxidized state (Hox) and one additional CO ligand in the inhibited state (Hox-CO). The diatomic ligands are sensitive reporter groups for… (More)
DOI: 10.1073/pnas.1606178113


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