Steps in the processing of Ad2 mRNA: Poly(A)+ Nuclear sequences are conserved and poly(A) addition precedes splicing

@article{Nevins1978StepsIT,
  title={Steps in the processing of Ad2 mRNA: Poly(A)+ Nuclear sequences are conserved and poly(A) addition precedes splicing},
  author={Joseph R. Nevins and James E. Darnell},
  journal={Cell},
  year={1978},
  volume={15},
  pages={1477-1493}
}
The conservation of nuclear Ad2 sequences during nucleocytoplasmic transport has been estimated from the accumulation of 3H-uridine in nuclear and cytoplasmic Ad2-specific RNA from the major late transcription unit. From 10-28% is conserved of the total Ad2 nuclear RNA synthesized from each of five regions of the genome that specify groups of 3' co-terminal mRNAs. The sum of the conservation of all the regions was equivalent to 100%, signifying the conservation of at least a part of each… 
Nuclear RNA is spliced in the absence of poly(A) addition
TLDR
Although poly(A) addition usually precedes splicing during mRNA formation, poly(B) is not required for splicing, and the approximately 2 kb RNA from Ad2 region E2 was shown to have sequences that lie more than 3.5 kb apart on the DNA, suggesting that correct cutting and splicing of the primary transcript in the absence of poly( A) synthesis had occurred.
Processing of late adenovirus nuclear RNA to mRNA. Kinetics of formation of intermediates and demonstration that all events are nuclear.
  • J. Nevins
  • Biology, Medicine
    Journal of molecular biology
  • 1979
TLDR
expression of the major late adenovirus transcription unit results in the production of five distinct poly(A)-containing RNAs, which are the initial precursors to one of five 3′ coterminal mRNA groups, and it would appear that all major processing steps occur in the nucleus.
Order of polyadenylic acid addition and splicing events in early adenovirus mRNA formation
TLDR
A study of the processing of mRNA from two early adenovirus type 2 transcription units revealed that polyadenylic acid [poly(A)] was added in most if not all cases to an unspliced nuclear RNA molecule whose coordinates extended from the apparent initiation site for RNA synthesis to the single poly(A) site in each transcription unit.
RNA Transcripts from the hsp7O Locus
Numerous protein-coding genes of the protozoan Trypanosoma brucei are arranged in tandem arrays that are transcribed polycistronically. The pre-mRNA transcripts are processed by trans splicing,
Are U4 small nuclear ribonucleoproteins involved in polyadenylation?
TLDR
It is found that the defined recognition sequence has elements which are complementary to regions within the small nuclear RNA U4, suggesting that U4 small nuclear ribonucleoproteins (snRNPs) may mediate polyadenylation in a fashion similar to the role of U1 snRNPs in splicing.
The Role of the poly(A) sequence in mammalian messenger RNA.
  • G. Brawerman
  • Chemistry, Medicine
    CRC critical reviews in biochemistry
  • 1981
TLDR
The poly(A) sequence is added to 3' termini of nuclear RNA segments destined to become part of the mRNA, and may play an essential role in the selection of these segments, and a role for this sequence in the promotion of mRNA stability is suggested.
Formation of the 3′ end of histone mRNA by post-transcriptional processing
The specific 3′ termini of a number of eukaryotic mRNAs have been shown to be generated by the post-transcriptional processing of primary transcripts or pre-mRNAs1–4. The sequence AAUAAA, present in
Formation of mRNA 3′ Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis
  • Jing Zhao, L. Hyman, C. Moore
  • Biology, Medicine
    Microbiology and Molecular Biology Reviews
  • 1999
TLDR
Recent findings suggest that the association of cleavage/polyadenylation factors with the transcriptional complex via the carboxyl-terminal domain of the RNA polymerase II (Pol II) large subunit is the means by which the cell restricts polyadenylations to Pol II transcripts.
Spliced exons of adenovirus late RNAs colocalize with snRNP in a specific nuclear domain
TLDR
The majority of the steady state viral RNA present in the nucleus is spliced at the tripartite leader exons, and it is suggested that viral RNA may accumulate in this late phase structure during an intranuclear step in RNA transport.
3′-End Cleavage and polyadenylation of nuclear Messenger RNA Precursors
In mammalian and probably all other eukaryotic cells, the 3′-ends of messenger RNAs are generated by post-transcriptional processing of longer precursors (reviewed in refs. 1–4). The pre-mRNA is
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