Step-wise cross-linking of pig heart lactate dehydrogenase by a heterobifunctional reagent.

@article{Kolkenbrock1978StepwiseCO,
  title={Step-wise cross-linking of pig heart lactate dehydrogenase by a heterobifunctional reagent.},
  author={H J Kolkenbrock and Hans H. Kiltz and Wolfgang E Trommer},
  journal={Biochimica et biophysica acta},
  year={1978},
  volume={535 2},
  pages={216-21}
}
Cross-linking of the essential cysteine-165 of pig heart lactate dehydrogenase (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27) with lysine-179 was achieved by stepwise reaction with N-(4-azidocarbonyl-3-hydroxy-phenyl)-maleimide. These two residues are connected by kind of a hydrophobic channel which suggests that the essential cysteine is not modified via the active center as previously assumed. 

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