Step changes in leaf oil accumulation via iterative metabolic engineering.

@article{Vanhercke2017StepCI,
  title={Step changes in leaf oil accumulation via iterative metabolic engineering.},
  author={Thomas Vanhercke and Uday K. Divi and Anna El Tahchy and Qing Liu and Madeline Mitchell and Matthew C Taylor and Peter J Eastmond and Fiona M. Bryant and A. A. Mechanicos and Cheryl A Blundell and Yao Zhi and Srinivas Belide and Pushkar Shrestha and Xue-Rong Zhou and Jean-Philippe F. Ral and Rosemary G White and Allan G. Green and Surinder Pal Singh and James Robertson Petrie},
  journal={Metabolic engineering},
  year={2017},
  volume={39},
  pages={237-246}
}
Synthesis and accumulation of plant oils in the entire vegetative biomass offers the potential to deliver yields surpassing those of oilseed crops. However, current levels still fall well short of those typically found in oilseeds. Here we show how transcriptome and biochemical analyses pointed to a futile cycle in a previously established Nicotiana tabacum line, accumulating up to 15% (dry weight) of the storage lipid triacylglycerol in leaf tissue. To overcome this metabolic bottleneck, we… CONTINUE READING
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