Stem-loop RT-PCR quantification of siRNAs in vitro and in vivo.

  title={Stem-loop RT-PCR quantification of siRNAs in vitro and in vivo.},
  author={Angie M. Cheng and Mu Li and Yu Liang and Yu Wang and Linda P W Wong and Caifu Chen and Alexander V Vlassov and Susan Magdaleno},
  volume={19 2},
RNA interference (RNAi) is a mechanism in which the introduction of small interfering RNAs (siRNAs) into a diverse range of organisms and cell types causes degradation of the complementary mRNA. Applications of RNAi include gene function and pathway analysis, target identification and validation, and therapeutics. There is a need to develop reliable and easy-to-use assays to evaluate siRNA delivery efficiency and distribution, study pathways, and stability of siRNAs in cells (posttransfection… CONTINUE READING