Stem-loop RT-PCR based quantification of small non-coding RNAs.

@article{Salone2015StemloopRB,
  title={Stem-loop RT-PCR based quantification of small non-coding RNAs.},
  author={V. Salone and Mathieu Rederstorff},
  journal={Methods in molecular biology},
  year={2015},
  volume={1296},
  pages={103-8}
}
Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse-transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions. 
Related Discussions
This paper has been referenced on Twitter 2 times. VIEW TWEETS

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…