Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours

@article{HoeiHansen2005StemCP,
  title={Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours},
  author={C. Hoei-Hansen and K. Almstrup and J E Nielsen and S. Brask Sonne and N. Graem and N. Skakkebaek and H. Leffers and E. Rajpert-De Meyts},
  journal={Histopathology},
  year={2005},
  volume={47}
}
Aims : NANOG is a key regulator of embryonic stem cell (ESC) self‐renewal and pluripotency. Our recent genome‐wide gene expression profiling study of the precursor of testicular germ cell tumours, carcinoma in situ testis (CIS), showed close similarity between ESC and CIS, including high NANOG expression. In the present study we analysed the protein expression of NANOG during normal development of human testis and in a large series of neoplastic/dysgenetic specimens. 
The pluripotency homeobox gene NANOG is expressed in human germ cell tumors
TLDR
The NANOG gene, a member of the homeobox family of DNA binding transcription factors, was recently identified in a screen for pluripotency‐promoting genes and expressed in human germ cells and tumors of germ cell origin. Expand
Origin of pluripotent germ cell tumours: The role of microenvironment during embryonic development
TLDR
Evidence supported by epidemiological studies indicate that disturbances in the hormonal microenvironment of the differentiating gonads may results in both the neoplasia and a host of other problems later in life, such as genital malformations, decreased spermatogenesis, and signs of hypogonadism. Expand
Expression and interdependencies of pluripotency factors LIN28, OCT3/4, NANOG and SOX2 in human testicular germ cells and tumours of the testis.
TLDR
Novel information is reported on the temporal and spatial expression pattern of LIN28 during normal human male germ-cell development as well as various types of GCTs, which suggests that LIN28 is associated with malignant behaviour of type II G CTs. Expand
Expression of Pluripotent Stem Cell Markers in the Human Fetal Testis
TLDR
Results suggest that PGCs maintain expression of pluripotent stem cell markers during and after sexual differentiation of the gonad, albeit in very low numbers. Expand
The pluripotency transcription factor Krüppel-like factor 4 is strongly expressed in intratubular germ cell neoplasia unclassified and seminoma.
TLDR
Examination of Gene Expression Omnibus data suggests that KLF4 may be an important factor for the maintenance of the developmental and the tumorigenic potential of IGCNU as well as for the malignancy of seminoma. Expand
Expression and Function of Pluripotency Genes in Adult Stem Cells
TLDR
This chapter discusses whether adult somatic stem cells express the Oct4 and Nanog genes that are known to play a role in the self-renewal and pluripotency characteristics of ESC, and whether such expression plays arole in the potency of adult tissue derived stem cells. Expand
NANOG promoter methylation and expression correlation during normal and malignant human germ cell development
TLDR
It is shown, that OCT3/4-SOX2 mediated expression of NANOG can be silenced by methylation of promoter CpG-sites, and global methylations of DNA decreased from fetal spermatogonia to mature sperm, which might reflect the cells need to suppress pluripotency in order to prevent malignant transformation. Expand
Analysis of SOX2 expression in developing human testis and germ cell neoplasia.
TLDR
SOX2 gene expression in CIS is demonstrated for the first time and the possibility of post-transcriptional regulation, most likely sumoylation as a mechanism for limiting SOX2 action in these cells is raised. Expand
Pluripotent Stem Cells from the Postnatal Testis: Unlocking the Potential of Spermatogonial Stem Cells
TLDR
The potential predisposing factors for postnatal germ cells to become pluripotent, including expression of pluripotency-associated genes and epigenetic factors are explored, and the data demonstrating functionality of the differentiated derivatives of ES-like cells are assessed. Expand
The Stem Cell Identity of Testicular Cancer
  • A. Clark
  • Biology, Medicine
  • Stem Cell Reviews
  • 2007
TLDR
The molecular pathways involved in human testis cancer will be presented based on research in human embryonic stem cells (hESCs), and also research using animal models, as well as the relationship between germ cell tumorigenesis and stem cell biology. Expand
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