Stem Cell Characteristics of Amniotic Epithelial Cells

  title={Stem Cell Characteristics of Amniotic Epithelial Cells},
  author={Toshio Miki and Thomas Lehmann and Hongbo Cai and Donna Beer Stolz and Stephen C Strom},
  journal={STEM CELLS},
Amniotic epithelial cells develop from the epiblast by 8 days after fertilization and before gastrulation, opening the possibility that they might maintain the plasticity of pregastrulation embryo cells. Here we show that amniotic epithelial cells isolated from human term placenta express surface markers normally present on embryonic stem and germ cells. In addition, amniotic epithelial cells express the pluripotent stem cell–specific transcription factors octamer‐binding protein 4 (Oct‐4) and… 

Stem Cells Derived from Human Fetal Membranes Display Multilineage Differentiation Potential

It is shown that only a very small proportion of primary hAECs contain class IA and class II human leukocyte antigens (HLAs), consistent with a low risk of tissue rejection, and suggest that the term amnion, an abundant and easily accessible tissue, may be a useful source of multipotent stem cells that possess a degree of immune privilege.

Identification of stem cell marker-positive cells by immunofluorescence in term human amnion.

Characterization of amniotic stem cells.

Assessment of the characterization of amnion-derived cells as stem cells is assessed by examining stemness marker expressions by flow cytometry, immunocytochemistry, and quantitative PCR.

Production of hepatocyte-like cells from human amnion.

It is reported that epithelial cells isolated from term placenta contain cells that express surface markers such as the stage-specific embryonic antigens (SSEA) and a gene expression profile that is similar to ES cells.

Amnion Epithelial Cells of Buffalo (Bubalus bubalis) Term Placenta Expressed Embryonic Stem Cells Markers and Differentiated into Cells of Neurogenic Lineage In Vitro

It is demonstrated that AE cells expressed pluripotent stem cell markers and have propensity to differentiate into cells of neurogenic lineage upon directed differentiation in vitro.

Amnion-derived pluripotent/multipotent stem cells

The unique characteristics of the AE stem cells are described and previous work concerning the stem cell nature of cells from amnion is summarized.

Quantitative comparison of stem cell marker-positive cells in fetal and term human amnion.

Human amniotic epithelial cells differentiate into cells expressing germ cell specific markers when cultured in medium containing serum substitute supplement

Human amniotic epithelial cells have the potential to differentiate into cells expressing germ cell specific markers, as well as the meiosis specific markers DMC1 and SCP3 at the protein level.

Derivation and characterization of human embryonic stem cells on human amnion epithelial cells

In the present work, inner cell masses were isolated from frozen embryos obtained as donations from couples undergoing in vitro fertilization (IVF) treatment and four new hESC lines were derived using primary human amnion epithelial cells as feeder cells.



Embryoid‐body cells derived from a mouse embryonic stem cell line show differentiation into functional hepatocytes

In vitro and in vivo experiments revealed that cultured EBs contain functional hepatocytes or hepatocyte‐like cells, suggesting that culturedEBs contain hepatocytes capable of producing ALB and urea.

Human Placenta‐Derived Cells Have Mesenchymal Stem/Progenitor Cell Potential

It is suggested that placenta‐derived cells have multilineage differentiation potential similar to MSCs in terms of morphology, cell‐surface antigen expression, and gene expression patterns.

Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro

The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.

A New Human Somatic Stem Cell from Placental Cord Blood with Intrinsic Pluripotent Differentiation Potential

A new, intrinsically pluripotent, CD45-negative population from human cord blood, termed unrestricted somatic stem cells (USSCs) is described, which grows adherently and can be expanded to 1015 cells without losing pluripotency.

Embryonic stem cell lines derived from human blastocysts.

Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate

Preimplantation Human Embryos and Embryonic Stem Cells Show Comparable Expression of Stage‐Specific Embryonic Antigens

It is shown that human ES cells are characterized by the expression of the cell‐surface antigens, SSEA3, S SEA4, TRA‐1‐60, and TRA‐ 1‐81, and by the lack of SSEA1, and that inner cell mass cells of the human blastocyst express a similar antigen profile, in contrast to the corresponding Cells of the mouse embryo.

Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research?

It is suggested that human amniotic fluid may represent a new source for the isolation of human Oct-4-positive stem cells without raising the ethical concerns associated with human embryonic research.

Human Placenta Feeder Layers Support Undifferentiated Growth of Primate Embryonic Stem Cells

Human placenta is found to be a useful source of feeder cells for the undifferentiated growth of primate ES cells on MEF feeders and produced typical immature teratomas in vivo after injection into severe combined immunodeficient mice.

Generation of Hepatocyte-Like Cells from Human Embryonic Stem Cells

It is reported here that hES cells can be induced to differentiate into hepatocyte-like cells, and these cells may provide a reliable source of normal human hepatocytes for research and transplantation.