Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries.

  title={Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries.},
  author={T. S. Wong and D. Roccatano and U. Schwaneberg},
  journal={Environmental microbiology},
  volume={9 11},
  • T. S. Wong, D. Roccatano, U. Schwaneberg
  • Published 2007
  • Biology, Medicine
  • Environmental microbiology
  • How to explore protein sequence space efficiently and how to generate high-quality mutant libraries that allow to identify improved variants with current screening technologies are key questions for any directed protein evolution experiment. High-quality mutant libraries can be generated through improved random mutagenesis methodologies and by restricting diversity generation through computational methods to residues which have high success probabilities. Advances in mutant library design and… CONTINUE READING
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