Staurosporine induces programmed cell death in embryonic neurons and activation of the ceramide pathway.

@article{Wiesner1996StaurosporineIP,
  title={Staurosporine induces programmed cell death in embryonic neurons and activation of the ceramide pathway.},
  author={Douglas A. Wiesner and G. E. Dawson},
  journal={Journal of neurochemistry},
  year={1996},
  volume={66 4},
  pages={1418-25}
}
We activated the death pathway in embryonic chick cerebral hemisphere neuron (E7CH) cultures with staurosporine (0.1-1.0 microM) and observed the meporphological changes, DNA laddering patterns, and DNA fragmentation (determined by Hoechst 33258 dye) associated with apoptosis. N-Acylsphingosine (C2-ceramide), a soluble ceramide analogue, was also able to induce apoptosis in these cells with the same characteristics and in the same time frame. We then observed that staurosporine was effective in… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 43 extracted citations

Similar Papers

Loading similar papers…