Status Report on the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures.

@article{Pap2018StatusRO,
  title={Status Report on the High-Throughput Characterization of Complex Intact O-Glycopeptide Mixtures.},
  author={Adam Pap and {\'E}va Klement and {\'E}va Hunyadi-Guly{\'a}s and Zsuzsanna Darula and Katalin F Medzihradszky},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2018},
  volume={29 6},
  pages={
          1210-1220
        }
}
A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra. Two search engines, Byonic and Protein Prospector, were used for the interpretation of… CONTINUE READING