Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins.

Abstract

This paper describes steps in the single-particle cryo-EM 3D structure determination of membrane proteins in their membrane environment. Using images of the Kv1.2 potassium-channel complex reconstituted into lipid vesicles, we describe procedures for the merging of focal-pairs of exposures and the removal of the vesicle-membrane signal from the micrographs… (More)
DOI: 10.1016/j.jsb.2016.01.012

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