Statistical design of quantitative mass spectrometry-based proteomic experiments.

@article{Oberg2009StatisticalDO,
  title={Statistical design of quantitative mass spectrometry-based proteomic experiments.},
  author={Ann L. Oberg and Olga Vitek},
  journal={Journal of proteome research},
  year={2009},
  volume={8 5},
  pages={
          2144-56
        }
}
We review the fundamental principles of statistical experimental design, and their application to quantitative mass spectrometry-based proteomics. We focus on class comparison using Analysis of Variance (ANOVA), and discuss how randomization, replication and blocking help avoid systematic biases due to the experimental procedure, and help optimize our ability to detect true quantitative changes between groups. We also discuss the issues of pooling multiple biological specimens for a single mass… 
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