Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042

@inproceedings{Bhunia2012StatisticalAF,
  title={Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042},
  author={Biswanath Bhunia and Apurba Dey},
  booktitle={Enzyme research},
  year={2012}
}
The optimization of physiochemical parameters for alkaline protease production using Bacillus licheniformis NCIM 2042 were carried out by Plackett-Burman design and response surface methodology (RSM). The model was validated experimentally and the maximum protease production was found 315.28 U using optimum culture conditions. The protease was purified using ammonium sulphate (60%) precipitation technique. The HPLC analysis of dialyzed sample showed that the retention time is 1.84 min with 73.5… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 32 references

A novel organic solventstable alkaline protease from organic solvent-tolerant Bacillus licheniformis YP1A

  • S. Li, B. He, Z. Bai, P. Ouyang
  • Journal of Molecular Catalysis B, vol. 56, no. 2…
  • 2009
Highly Influential
6 Excerpts

Extracellular alkaline protease from Bacillus licheniformis NCIM-2042: improving enzyme activity assay and characterization

  • B. Bhunia, D. Dutta, S. Chaudhuri
  • Engineering in Life Sciences, vol. 11, no. 2, pp…
  • 2011
Highly Influential
3 Excerpts

Purification and characterization of a solvent, detergent and oxidizing agent tolerant protease from Bacillus cereus isolated from the Gulf of Khambhat

  • K. Shah, K. Mody, J. Keshri, B. Jha
  • Journal of Molecular Catalysis B, vol. 67, no. 1…
  • 2010
2 Excerpts

Biochemical and molecular characterization of a detergent-stable serine alkaline protease from Bacillus pumilus CBS with high catalytic efficiency

  • B. Jaouadi, S. E. Chaabouni, M. Rhimi, S. Bejar
  • Biochimie, vol. 90, no. 9, pp. 1291–1305, 2008.
  • 2008
2 Excerpts

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