Static light scattering to characterize membrane proteins in detergent solution.

@article{Slotboom2008StaticLS,
  title={Static light scattering to characterize membrane proteins in detergent solution.},
  author={Dirk Jan Slotboom and Ria H Duurkens and Kees Olieman and Guus Bjorn Erkens},
  journal={Methods},
  year={2008},
  volume={46 2},
  pages={73-82}
}
Determination of the oligomeric state or the subunit stoichiometry of integral membrane proteins in detergent solution is notoriously difficult, because the amount of detergent (and lipid) associated with the proteins is usually not known. Only two classical methods (sedimentation equilibrium centrifugation and static light scattering) can measure directly the absolute molecular mass of a protein present in a protein/detergent micelle, without any assumption on the amount of detergent bound, or… CONTINUE READING

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