State of actin cytoskeleton and development of slow-frozen and vitrified rabbit pronuclear zygotes.

@article{Kulkov2016StateOA,
  title={State of actin cytoskeleton and development of slow-frozen and vitrified rabbit pronuclear zygotes.},
  author={B Kul{\'i}kov{\'a} and Estrella Jim{\'e}nez-Trigos and Alexander V Makarevich and Peter Chrenek and Jos{\'e} Salvador Vicente and Francisco Marco-Jim{\'e}nez},
  journal={Cryobiology},
  year={2016},
  volume={72 1},
  pages={
          14-20
        }
}
This study was focused on the effect of cryopreservation on the state of actin cytoskeleton and development of rabbit pronuclear zygotes. Zygotes were collected from superovulated females and immediately used for 1) slow-freezing in a solution containing 1.5 M 1,2-propanediol and 0.2 M sucrose, or 2) vitrification in a solution containing 42.0% (v/v) of ethylene glycol, 18.0% (w/v) of dextran and 0.3 M sucrose as cryoprotectants. After thawing or warming, respectively, zygotes were evaluated… CONTINUE READING
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