Starch determination in Chlorella vulgaris—a comparison between acid and enzymatic methods

  title={Starch determination in Chlorella vulgaris—a comparison between acid and enzymatic methods},
  author={Bruno Jos{\'e} Dum{\^e}t Fernandes and Giuliano M Dragone and Ana Patr{\'i}cia Abreu and Pedro Geada and Jos{\'e} Ant{\'o}nio Couto Teixeira and Ant{\'o}nio Augusto Vicente},
  journal={Journal of Applied Phycology},
Different methods for estimating starch in Chlorella vulgaris were compared with the view of establishing a procedure suitable for rapid and accurate determination of starch content in this microalgal species. A close agreement was observed between methods that use perchloric acid and enzymatic methods that use α-amylase and amyloglucosidase to hydrolyze the starch of microalgae grown under different nitrogen culture conditions. Starch values obtained by these methods were significantly higher… CONTINUE READING
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