Staphylococcal alpha-toxin: the role of the N-terminus in formation of the heptameric pore -- a fluorescence study.

@article{Valeva1997StaphylococcalAT,
  title={Staphylococcal alpha-toxin: the role of the N-terminus in formation of the heptameric pore -- a fluorescence study.},
  author={Angela Valeva and J Pongs and Sucharit Bhakdi and Michael Palmer},
  journal={Biochimica et biophysica acta},
  year={1997},
  volume={1325 2},
  pages={281-6}
}
Staphylococcus aureus alpha-toxin forms heptameric pores on eukaryotic cell membranes. Assembly of the heptamer precedes formation of the transmembrane pore. The latter event depends on a conformational change that drives a centrally located stretch of 15 amino acid residues into the lipid bilayer. A second region of the molecule that has been implicated in the pre-pore to pore transition is the far N-terminus. Here, we used fluorescently labeled single cysteine replacement mutants to analyze… CONTINUE READING
Highly Cited
This paper has 26 citations. REVIEW CITATIONS