Standardized assay medium to measure Lactococcus lactis enzyme activities while mimicking intracellular conditions.

@article{Goel2012StandardizedAM,
  title={Standardized assay medium to measure Lactococcus lactis enzyme activities while mimicking intracellular conditions.},
  author={Anisha Goel and Filipe Santos and Willem M. de Vos and Bas Teusink and Douwe Molenaar},
  journal={Applied and environmental microbiology},
  year={2012},
  volume={78 1},
  pages={134-43}
}
Knowledge of how the activity of enzymes is affected under in vivo conditions is essential for analyzing their regulation and constructing models that yield an integrated understanding of cell behavior. Current kinetic parameters for Lactococcus lactis are scattered through different studies and performed under different assay conditions. Furthermore, assay conditions often diverge from conditions prevailing in the intracellular environment. To establish uniform assay conditions that resemble… CONTINUE READING

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